JNTU
JNTU B.Pharmacy IV INSTRUMENTAL METHODS OF ANALYSIS Previous Question Paper (January/February - 2023 - R17)
Question paper consists of Part A, Part B.
Part A is compulsory, which carries 25 marks. In Part A, answer all questions.
In Part B, Answer any one question from each unit. Each question carries 10 marks and may have a, b as sub questions.
Part A
Create the Jablonski Energy Diagram.
Answer
The Jablonski diagram illustrates the electronic energy states of a molecule and the transitions between them. It represents the ground state (S0) and excited singlet states (S1, S2) and triplet states (T1, T2) as horizontal lines. Vertical arrows indicate transitions: upward arrows represent absorption, while downward arrows represent radiative processes such as fluorescence (S1 to S0) and phosphorescence (T1 to S0). Non-radiative processes like internal conversion and intersystem crossing are represented by horizontal or wavy lines between states.
Generate the equation of Beer-Lambert’s Law.
Answer
Beer-Lambert’s Law describes the linear relationship between the absorbance of a solution and its concentration. It is derived by combining Beer’s Law, which states that absorbance is directly proportional to concentration, and Lambert’s Law, which states that absorbance is directly proportional to the path length of the sample.
The mathematical equation is:
Where:
- A is the absorbance (dimensionless).
- ε (epsilon) is the molar absorptivity or molar extinction coefficient (L mol⁻¹ cm⁻¹).
- b is the path length of the sample cell or cuvette (usually in cm).
- c is the concentration of the absorbing species (mol L⁻¹).
Justify the reason on which types of sample undergoes Atomic Absorption Spectroscopy.
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Assess the principle involved in Nepheloturbidometry.
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How do you interpret the Rf value in Chromatography.
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How do you develop a TLC plate using Silica gel at lab conditions?
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Support the statement “HPLC (High Performance Chromatography) is also known as High Pressure Chromatography.
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Assess and tell why and how temperature programming is done in GC.
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Justify on the principle involved in Gel Chromatography.
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Generate the physical properties of Ion exchange resins.
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Part B
Define the terms ‘Chromophore’ and ‘Auxochrome’. Compare and describe about various shifts involved in UV-Visible spectroscopy by constructing a neat graph.
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List and explain on various types of Quenching involved in Fluorescence.
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Demonstrate and explain the instrumentation involved in Fluorimetry.
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List the applications of UV-Visible spectroscopy. Summarize on Single and multi-Component analysis.
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Demonstrate on the procedure involved in sample preparation of solids and liquids for IR Spectroscopic analysis.
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Describe the construction and working of Duboscq Colorimeter involved in Nephelometer.
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Explain in detail the various factors involved in IR spectroscopy.
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Construct the block diagram of Internal Standard Flame Photometer and explain its working.
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Compare the Advantages and Disadvantages of TLC with that of Paper Chromatography.
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Summarize on the procedure involved in Gel Electrophoresis.
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Categorize and describe on various types of development techniques involved in Paper Chromatography.
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List the types and summarize the Applications on Electrophoresis.
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Explain in detail various derivatization techniques involved in Gas Chromatography.
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Enumerate the applications of HPLC.
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List out the various detectors involved in HPLC. Describe the Construction and Working of UV detector.
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Construct only the block diagram of Gas Chromatography Instrument. List the ideal properties of Mobile Phase involved in Gas Chromatography.
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Contrast the difference between Gel Filtration and Gel Permeation Chromatography. List the stationary phases involved in Gel Chromatography.
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Describe the working principle of Affinity Chromatography.
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Describe on Factors affecting Ion-exchange separations.
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List the applications of Affinity Chromatography.
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