JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD B.Pharmacy IV Year INSTRUMENTAL METHODS OF ANALYSIS Previous Question Paper (June/July - 2024 - R17)

B.Pharmacy | IV Year | IV-I | R17 | June/July - 2024

Answer PaperPaper Code: 247AAMaximum Marks: 75Time: 3 Hours

i) The question paper consists of Part A and Part B.

ii) Part A is compulsory, which carries 25 marks. In Part A, answer all questions.

iii) In Part B, answer any one question from each unit. Each question carries 10 marks and has a and b as sub-questions.

PART- A

125 marks

Define Chromophores and Auxochromes giving examples.

2 marks

Answer

Chromophores are unsaturated functional groups capable of absorbing radiation, resulting in electronic transitions (e.g., C=C, C=O, -N=N-). Auxochromes are saturated groups containing lone pairs of electrons that, when attached to a chromophore, shift the absorption maximum to longer wavelengths (bathochromic shift) and increase intensity (e.g., -OH, -NH2, -OCH3).

Explain the difference between singlet and triplet excited states of electrons, and indicate which is associated with the emission of fluorescent radiation.

3 marks

Answer

In a singlet state, the electron spins are paired (antiparallel), whereas in a triplet state, the electron spins are parallel. Fluorescence is associated with the transition from the first excited singlet state (S1) back to the ground singlet state (S0).

What are the advantages of FTIR equipment over that of dispersive type?

2 marks

Answer

Describe the special feature associated with the radiation source used in an Atomic Absorption Spectroscopy technique.

3 marks

Answer

Define and indicate the significance of Rf values.

2 marks

Answer

Describe the methodology and applications of the TLC technique.

3 marks

Answer

Describe the properties of the stationary phase used in a GLC technique.

2 marks

Answer

Name and explain the working principle of any one type of in-line detector used in HPLC equipment.

3 marks

Answer

What is the basic principle involved in the separation of a compound from the sample by the Gel Chromatography technique?

2 marks

Answer

What is the purpose of using a suppressor column in the analysis of eluate in the Ion-exchange chromatography technique and how do they act?

3 marks

Answer

PART-B

210 marks

Describe the instrumental components of a UV-visible spectrophotometer and explain the working principle of the equipment.

5 marks

Answer

Define Quencing giving examples and explain how this phenomenon is useful in the quantitative analysis of some substances by the Fluorimetery technique.

5 marks

Answer

310 marks

Describe the utility of Beer-Lambert’s equation in the quantitative analysis of medicinal compounds citing examples from the Indian Pharmacopoeia.

5 marks

Answer

Explain the working principle of a Filter Fluorimeter giving a schematic diagram of the instrumentation.

5 marks

Answer

410 marks

Explain the working principle ATR-IR technique and indicate the advantages of this technique over the conventional method of sample handling.

5 marks

Answer

Describe the interferences associated with the analysis of samples by Flame Photometry and mention the necessary measures to overcome such interferences.

5 marks

Answer

510 marks

Name two detectors used in IR Spectrometers and explain their working principle giving necessary schematic diagrams.

5 marks

Answer

Explain the principle of Nephelometry and Turbidometry giving schematic diagrams, and describe their applications citing examples.

5 marks

Answer

610 marks

Classify the various techniques of chromatography based on different considerations giving an example of each type.

5 marks

Answer

Explain the working principle and describe the applications of the Gel Electrophoresis technique giving examples.

5 marks

Answer

710 marks

Explain the distinctive features between Adsorption Column Chromatography and Partition Column Chromatography and describe their applications.

5 marks

Answer

Describe the similarities and differences between Paper Chromatography and Paper Electrophoresis in the working principle and their applications.

5 marks

Answer

810 marks

Describe the major changes made in the conventional liquid chromatography to increase the performance of the technique resulting the High-Performance Liquid Chromatography (HPLC) technique.

5 marks

Answer

Explain the working principle of a Gas Chromatograph giving a schematic diagram of instrumentation.

5 marks

Answer

910 marks

Describe the instrumentation of a High-Performance Liquid Chromatography equipment giving a block diagram.

5 marks

Answer

Why and how derivatization of certain samples are done for their analyses by Gas Chromatography?

5 marks

Answer

1010 marks

What are ion-exchange resins and how they are modified for ion-exchange processes in the Ion-exchange Chromatography?

5 marks

Answer

Explain the working principle and methodology adopted in a Gel Chromatography technique giving an example.

5 marks

Answer

1110 marks

Explain the basic principle involved, and describe the advantages and limitations of the Affinity Chromatography technique.

5 marks

Answer

Name the various methods available for the detection of analyte in the Ion-exchange Chromatography and explain the working principle of any one method.

5 marks

JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD B.Pharmacy IV Year INSTRUMENTAL METHODS OF ANALYSIS Previous Question Paper (June/July - 2024 - R17)

PART- A

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PART-B

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