JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

The metabolism of complex biochemical substances are discussed in

detail.The Biochemical organization and Bioenergetics which will help the students to understand the concepts of biochemistry.

Course Outcome

The metabolism of complex biochemical compounds would make the

students to gain a good knowledge about biochemical organization in the human system.

UNIT - I

• (a) Biochemical organization of the cell, molecular constitutents of membrane, active & passive transport process, sodium and potassium pumps, osmoregulation and heamostatis.
• (b) Bio-energetics: The concept of free energy, laws of thermodynamics.Determination of change in free energy from equilibrium constant & reduction potential.
• (c) The respiratory chain & its role in energy capture & its control. Oxidative phosphorylation & its energetics & Electron Transport Chain, mechanism of actions. Production of ATP and its biological significance

UNIT - II

Enzymes & Co-enzymes: Classification, Structure, mechanism of action, properties, factors affecting enzymes action. Activators & de activators of enzymes, enzyme kinetics & enzyme inhibitions, repressions with reference to drug action.

UNIT - III

Metabolism of Carbohydrates: Biochemistry of carbohydrates, Glycolysis, glycogenesis, glycogenolysis, gluconeogenesis, Kreb’s cycle, HMP shunt & uronic acid pathways, anaerobic respiration in muscle.

UNIT - IV

Metabolism of Proteins: Biochemistry of proteins, Amino acid structure & classifications, de amination, Trans-amination, de-carboxylation, Urea cycle, Metabolism of valine, cystine, cystein, tryptophan, tyrosine, methionine.

UNIT - V

• a) Metabolism of Lipids: Biochemistry of lipids, Alpha, Beta, Gama & Omega oxidations of fatty acids, bio- synthesis of fatty acids, cholesterol, ketogenesis.
• b) Introduction to xenobiotic metabolism, detoxification mechanisms, biochemistsry and metabolism of nucleic acids and vitamins.

TEXT BOOKS

• 1. Harper’s Biochemistry
• 2. A.L.Lehninger, Principles of Biochemistry.
• 3. Satyanarayana, Text Book of Biochemistry

REFERENCES

• 1. L.Stryer, Text Book of Bio Chemistry.
• 2. E.E Conn & P.K. Stumpf, Outlines of Biochemistry by, Publ, John Wiley & sons, New York.