JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

The basic concepts and analytical techniques of various

pharmaceuticals are discussed in a detailed manner.

Course Outcome

The knowledge gained upon the detailed study of the analytical

techniques will be useful to analyze the pharmaceutical substances in a systematic qualitative and quantitative manner.

UNIT - I

• a) Computation of analytical results, significant figures, concept of error, precision, accuracy, standard deviation, rejection of doubtful values with special reference to volumetric analysis. Calibration of analytical equipment used in volumetric analysis.
• b) Theory of Neutralization Titrations: Acid-base concept, Acidimetry, Alkalimetry, Common ion effect and solubility product, pH, buffers and indicators.
• c) General principles and theory of oxidation-reduction methods and precipitation methods. An account of the indicators used in these titrations. Application of the above methods in the analysis of drugs, as under IP 2010

UNIT - II

• a) Complexometric titration: Theory, types and application in pharmaceutical analysis. Masking and demasking and their applications.
• b) Non-aqueous titration: Theory, types, solvents used and application in pharmaceutical analysis.
• c) Gravimetry: Principles, Theory, Precipitation, co-precipitation and applications

UNIT - III

• a) Potentiometry: Introduction, electrochemical cells and half cells. Electrode, measurement of potential, applications in pharmaceutical analysis.
• b) Conductrometric titrations. Basic concepts, different types of conductrometric titrations, apparatus used, applications in pharmaceutical analysis.
• c) Polarography: Basic concepts, apparatus and principles, general polarographic analysis, applications in Pharmaceutical Analysis.
• d) Amperometric Titrations

UNIT - IV

Study of separations and determinations involving the following techniques and their applications in pharmacy

• a) Coloumn chromatography; Adsorption and partition theory, preparation, procedure, methods of detection.
• b) Thin layer chromatography: theoretical consideration, preparation, procedure, detection of compounds.
• c) Paper Chromatography: theory of partition, different techniques employed filter papers used, quantitative and quantitative detection.

UNIT - V

• a) Flamephotometry: Introduction, study and working priciples of instrumentations used for analysis, applications in pharmaceutical analysis.
• b) Principle, instrumentation and applications involved in the follwing
• i. Refractometry ii. Polarimetry iii. Nephlometry and turbidimetry
• c) Physical and chemical methods of determination of moisture content (including Karl-Fisher method).

TEXT BOOKS:

• 1. Skoog-Instumental Analysis and Skoog fundamentals of analytical Chemistry
• 2. A.H. Beckett & J.B Stanlake Vol.I&II., Practical Pharmaceutical Chemistry, Athlone Press of the Univ of London
• 3. Chatwal & Anand, Instrumental Methods of Analysis.

REFERENCES:

• 1. A.I Vogel, Quantitative Chemical Analysis, ELBS ed.
• 2. B.K. Sarma, Instrumental Chemical Analysis, Goel Publishers