JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD
PS307: PHARMACOGNOSY – I LAB
B.Pharmacy II Year I Semester (R16)
Overview
1. Introduction to the materials required for microscopic work, preparation of histological slides and their focusing to obtain the critical illumination with the instructions for the use of microscope.
2. Preparation of commonly used reagents in microscopic work.
3. Identification of following cell contents in plant materials by microscopical and microchemical tests: Starch grains in potato, maize, rice and wheat.
4. Identification of following cell contents in plant materials by microscopical and microchemical tests
5. Mucilage
6. Aleurone grains
7. Fixed oils
8. Measurement of dimensions of cells and cell contents. Introduction to micrometer and camera lucida (drawing occular).Measurement of dimensions of starch grains in powdered ginger.
9. Identification of cinnamon by measuring the dimensions of starch grains
10. Detection/ identification of Carbohydrates by chemical tests.
11. Detection/ identification of lipids by chemical tests.
12. Isolation of starch from Potato.
13. Determination of Swelling factor in crude drugs
14. Identification of crude drugs mentioned in the theory by Organoleptic method.
15. Identification test for Tannins.
16. Identification test for Resins.
17. Determination of volatile oils content of Eucalyptus leaf or Fennel by using Clevenger apparatus
18. Determination of Eugenol content in clove oil and detection by TLC.
REFERENCES:
1. Kandhelwal, Practical Pharmocognosy.
2. C.K. Kokate et.al, Practical Pharmocognosy.
3. Iyengar, Practial Pharmocognosy
4. Practical Pharmaognosy, Dr. V. Duraiswamy, Dr. K.N. Jayaveera.
5. Anatomy of Crude Drugs by M.A.Iyengar and S.C.K.Nayak – 12th Edition
6. Practical Pharmacognosy by Dr. G.S. Kumar and Dr. K.N. Jayaveera
7. Practical Pharmacognosy by Saroja Joshi and Vidhu Aeri