JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

PS508: PHARMACEUTICAL MICROBIOLOGY LAB

B.Pharmacy III Year I Semester (R16)

Overview

  • 1. Introduction to equipment and glassware used in microbiology laboratory.
  • 2. Study of morphology of different microbes
  • 3. Preparation of various culture media, cultivation of microbes and observation of colony characteristics.
  • 4. Sterilization techniques (moist and dry heat) and their validations.
  • 5. Aseptic transfer of culture into different types of media.
  • 6. Characterisation of microbes by staining techniques (simple, gram’s, acid fast and negative staining).
  • 7. Study of motility of bacteria by hanging drop method.
  • 8. Characterization of microbes through Bio chemical reactions:
  • a. Indole test.
  • b. Methyl red test.
  • c. Voges proskauer test.
  • d. Starch hydrolysis test.
  • e. Fermentation of carbohydrates.
  • 9. Isolation of pure cultures by streak plate, spread plate & pour plate techniques.
  • 10. Enumeration of bacteria by pour plate/spread plate technique
  • 11. Enumeration of bacteria by direct microscopic count.
  • 12. Evaluation of any disinfectant by phenol coefficient test
  • 13. Study of Oligodynamic action (of metals on bacteria)
  • 14. Preservation of microrganisms (slant and stab cultures)
  • 15. Microbiological Analysis of Water.

REFERENCE

  • 1. Garg,F C Experimental Microbiology
  • 2. Gaud.R.S, Gupta G.D, Practical Microbiology
  • 3. Vanitha Kale and kishore Bhusari, Pratical microbiology principles and Techniques

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