JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Overview

• 1. Introduction to Experimental Pharmacology
• a) Preparation of different solutions for experiments.
• b) Drug dilutions, use of molar and w/v solutions in experimental Pharmacology.
• c) Common laboratory animals and anesthetics used in animal studies.
• d) Commonly used instruments in experimental pharmacology.
• e) Some common and standard techniques.
• f) Bleeding and intravenous injection, intragastric administration.
• 2. Experiments on intact preparations: Study of different routes of administration of drugs in mice/rats.
• 3. Experiments in Central Nervous system: Recording of spontaneous motor activity, stereotype, analgesia, anticonvulsant activity, anti-inflammatory activity,
• 4. To study the effect of autonomic drugs on rabbit’s eye
• 5. Experiments on Isolated Preparations: To study the effects of various agonists and antagonists and their characterisation using isolated preparations like frog's rectus abdominus muscle and isolated ileum preparation of rat& guinea pig.
• a) To record the concentration response curve (CRC) of acetylcholine using rectus abdominus muscle preparation of frog.
• b) To study the effects of physostigmine and d-tubocurarine on the CRC of acetylcholine using frog rectus abdominus muscle preparation of frog.
• c) To record the CRC of 5-HT on rat fundus preparation.
• d) To record the CRC of histamine on guineapig ileum preparation.
• e) To study the inotropic and chronotropic effects of drugs on isolated frog heart.
• f) To study the effects of drugs on normal and hypodynamic frog heart.
• 6. Experiments pertaining to analgesia, anti-convulsant activity, anti-inflammatory activity (Only demonstration).

REFERENCE:

Experimental Pharmacology, M.C. Prabhakar.