JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Overview

. Experiments on Isolated Preparations:

• a. To calculate the PA2 value of atropine using acetylcholine as an agonist on rat ileum preparation.
• b. To calculate the PA2 value of mepyramine or chlorampheniramine using histamine as agonist on guinea pig ileum.
• c. To find out the strength of the given sample on (e.g. Acetycholine, Histamine, 5-HT. Oxytocin etc.) Using a suitable isolated muscle preparation by
• i. Matching Assay
• ii. Two point Assay
• iii. Three point Assay
• 2. Pharmacology of the Gastrointestinal Tract To study the anti-secretory and anti-ulcer activity using pylorus ligated rats.