JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

The principles involved in the determination of various bulk drugs and

formulations are discussed. Modern methods and instrumental techniques are applied in the study and analysis of pharmaceutical substances.

Course Outcome

The students are exposed to the modern instrumental techniques for the study of

pharmaceuticals to a high level which would be useful for their future in academia and industry.

UNIT - I

• a. UV & Visible Spectrophotometry: Introduction to Spectroscopy, Basic terminology – Chromophore, Auxochrome, Bathochromic shift, Hypsochromic shift, hyperchromic and hypochromic shift. UV & Visible Spectrophotometry: Principle, Theory, Beer-Lamberts Law & Deviations, Instrumentation – Single Beam and Double Beam Spectrophotometers, Applications, Woodward –Feiser rule.
• b. Flourimetry: Principle, Theory, Quenching, Instrumentation and applications.

UNIT - II

• a. Infrared Spectrophotometry (IR): Introduction, principle, theory, types of vibrations, Instrumentation, Single and double beam spectrophotometer, sampling techniques, applications, basic principles in the interpretation of IR Spectra.
• b. Atomic Absorption Spectroscopy: Principle, Theory, Instrumentation and applications.

UNIT - III

Nuclear Magnetic Resonance Spectrophotometry (NMR) : Basic Principle, theory, instrumentation, chemical shift, shielding and deshielding, relaxation processes, spin-spin splitting, applications, basic principles in the interpretation of NMR spectra.

UNIT - IV

Mass Spectrometry: Basic principle, theory, instrumentation and applications, basic principles in the interpretation of Mass Spectra.

UNIT - V

An Elementary study of the following:

• (a) GC: Columns, Carrier gas and detectors used
• (b) HPLC & HPTLC: Basic Principles
• (c) Electrophoresis: Various types of Electrohoresis
• (d) ORD Curves, RIA & ELISA: Basic principles

TEXT BOOKS

• 1. R.M. Silvesterin and G.C. Bassler.Spectrometric Identification of Organic Compounds.
• 2. AH Beckett & Stenlake, Text book of Practical Pharmaceutical chemistry, Vol.I&II
• 3. AI Vogel, Quantitative Chemical Analysis.

REFERENCES

• 1. Settle, Handbook of Instrumental Techniques for Analytical Chemistry.
• 2. Y.Anjaneyulu & Maraiah, Quality Assurance & Quality Management in Pharmaceutical Industry. R16 B.PHARMACY IV YEAR