JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

Pharmaceutical biotechnology is considered to be a logical extension of

pharmaceutical microbiology, thus expected to show a dramatic change in the drug product scenario in future. This course is designed to impart knowledge on isolation of industrially interesting microbes, various techniques employed in biotechnology Viz., r-DNA technology, Hybridoma technology, enzyme technology and the products derived using these techniques.

Course Outcome

Upon completion of the course, the student shall be able to-

• Know screeing of industrially interesting microbes.
• Optimize fermentation process parameters
• Know about preparation, standardization, storage and labelling of biotechnologically derived products
• Know about bioinformatics and its applications in pharmacy.
• Know about the regulatory control of biotechnological products.

UNIT - I

• a. Fermentation Technology: Isolation, Selection and Screening of Industrially important microbes, Strain improvement. Types of fermentations, optimization of fermentation process.Types, design & operation of Bioreactor.
• b. Specific Fermentations: Selection of organism, fermentation & purification of various antibiotics, vitamins, aminoacids, organic acids, solvents, biomass like penicillin, streptomycin, tertacycline, erythromycin, cyanocobalamin, glutamic acid, citric acid, alcohol, Lactobacillus sporogenes.

UNIT - II

• a. Recombinant DNA Technology: Introduction to r-DNA technology and genetic engineering, steps involved in isolation of enzymes, vectors, recombination and cloning of genes. Production of r-DNA technology derived therapeutic proteins like humulin, humatrope, intron a, recombivax HB(hepatitis b).
• b. Hybridoma Technology: Production and applications of Monoclonal Antibodies.

UNIT - III

Immunology & Immunological Preparations: Principles of Immunity, Humoral immunity, cell mediated immunity, Antigen – Antibody reactions, Hypersensitivity reactions. Active & passive immunizations preparation of vaccines, standardization & storage of BCG, cholera, smallpox, polio, typhus, tetanus toxoid, immuno serum & diagnostic agents.

UNIT - IV

• a. Enzyme Technology: Methods of immobilization of enzymes and cells and their applications, factors affecting immobilized enzyme kinetics, advantages of immobilized enzymes over isolated enzymes.Study of enzymes such as hyaluronidase, penicillinase, streptokinase & streptodornase, protease.
• b. Blood Products: Collection processing, storage and control of official blood products, plasma substitutes (dextran) and sutures & ligatures.

UNIT - V

• a. Microbial Transformations: Types, Methods of bioconversions & Application in Pharma Industry, Steroidal transformations. R16 B.PHARMACY IV YEAR
• b. An introductory study on bioinformatics and its appilications, Regulatory control of Biotechnological products.

TEXT BOOKS

• 1. P. F. Stanbury & A. Whitaker, Principles of fermentation technology, Pergamon Press
• 2. Sambamurthy. K, Text Book of Pharmaceutical Biotechnology.
• 3. S. S. Kori, Pharmaceutical biotechnology.

REFERENCES

• 1. Wulf Crueger and Anneliese Crueger, Biotechnology, 2nd Ed, Publ- Panima publication co- peration, New Delhi
• 2. U. Satyanarayana, Text book of Biotechnology R16 B.PHARMACY IV YEAR