JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

List of Experiments:

• 1. Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology.
• 2. Sterilization of glassware, preparation and sterilization of media.
• 3. Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations.
• 4. Staining methods- Simple, Grams staining and acid fast staining (Demonstration with practical).
• 5. Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques.
• 6. Microbiological assay of antibiotics by cup plate method and other methods
• 7. Motility determination by Hanging drop method.
• 8. Sterility testing of pharmaceuticals.
• 9. Bacteriological analysis of water
• 10. Biochemical test (IMViC reactions)
• 11. Revision Practical Class Recommended Books (Latest edition)
• 1. W.B. Hugo and A.D. Russel: Pharmaceutical Microbiology, Blackwell Scientific publications, Oxford London.
• 2. Prescott and Dunn., Industrial Microbiology, 4th edition, CBS Publishers & Distributors, Delhi.
• 3. Pelczar, Chan Kreig, Microbiology, Tata McGraw Hill edn.
• 4. Malcolm Harris, Balliere Tindall and Cox: Pharmaceutical Microbiology.
• 5. Rose: Industrial Microbiology.
• 6. Probisher, Hinsdill et al: Fundamentals of Microbiology, 9th ed. Japan
• 7. Cooper and Gunn’s: Tutorial Pharmacy, CBS Publisher and Distribution.
• 8. Peppler: Microbial Technology.
• 9. I.P., B.P., U.S.P.- latest editions.
• 10. Ananthnarayan : Text Book of Microbiology, Orient-Longman, Chennai
• 11. Edward: Fundamentals of Microbiology.
• 12. N.K.Jain: Pharmaceutical Microbiology, Vallabh Prakashan, Delhi
• 13. Bergeys manual of systematic bacteriology, Williams and Wilkins- A Waverly company