JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives:

• Biotechnology has a long promise to revolutionize the biological sciences and technology.
• Scientific application of biotechnology in the field of genetic engineering, medicine and fermentation technology makes the subject interesting.
• Biotechnology is leading to new biological revolutions in diagnosis, prevention and cure of diseases, new and cheaper pharmaceutical drugs.
• Biotechnology has already produced transgenic crops and animals and the future promises lot more.
• It is basically a research-based subject.

Course Outcomes

Upon completion of the subject student shall be able to;

• Understanding the importance of Immobilized enzymes in Pharmaceutical Industries
• Genetic engineering applications in relation to production of pharmaceuticals
• Importance of Monoclonal antibodies in Industries
• Appreciate the use of microorganisms in fermentation technology

UNIT - I

• a. Brief introduction to Biotechnology with reference to Pharmaceutical Sciences.
• b. Enzyme Biotechnology- Methods of enzyme immobilization and applications.
• c. Biosensors- Working and applications of biosensors in Pharmaceutical Industries.
• d. Brief introduction to Protein Engineering.
• e. Use of microbes in industry. Production of Enzymes- General consideration - Amylase, Catalase, Peroxidase, Lipase, Protease, Penicillinase.
• f. Basic principles of genetic engineering.

UNIT - II

• a. Study of cloning vectors, restriction endonucleases and DNA ligase.
• b. Recombinant DNA technology. Application of genetic engineering in medicine.
• c. Application of r DNA technology and genetic engineering in the products:
• d. Interferon b) Vaccines- hepatitis- B c) Hormones- Insulin.
• e. Brief introduction to PCR Types of immunity- humoral immunity, cellular immunity

UNIT - III

• a. Structure of Immunoglobulins
• b. Structure and Function of MHC
• c. Hypersensitivity reactions, Immune stimulation and Immune suppressions.
• d. General method of the preparation of bacterial vaccines, toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other products relative to immunity.
• e. Storage conditions and stability of official vaccines
• f. Hybridoma technology- Production, Purification and Applications

UNIT - IV

• a. Immuno blotting techniques- ELISA, Western blotting, Southern blotting.
• b. Genetic organization of Eukaryotes and Prokaryotes
• c. Microbial genetics including transformation, transduction, conjugation, plasmids and transposons. R17 B. PHARMACY III YEAR
• d. Introduction to Microbial biotransformation and applications.
• e. Mutation.

UNIT - V

• a. Types of mutation/mutants
• b. Fermentation methods and general requirements, study of media, equipments, sterilization methods, aeration process, stirring.
• c. Large scale production fermenter design and its various controls.
• d. Study of the production of - penicillins, citric acid, Vitamin B12, Glutamic acid, Griseofulvin, Recommended Books (Latest edition):
• 1. B.R. Glick and J.J. Pasternak: Molecular Biotechnology: Principles and Applications
• 2. of Recombinant DNA: ASM Press Washington D.C.
• 3. RA Goldshy et. al., Kuby Immunology.
• 4. J. W. Goding: Monoclonal Antibodies.
• 5. J.M. Walker and E.B. Gingold: Molecular Biology and Biotechnology by Royal Society of Chemistry.
• 6. Zaborsky: Immobilized Enzymes, CRC Press, Degraland, Ohio.
• 7. S.B. Primrose: Molecular Biotechnology (Second Edition) Blackwell Scientific Publication.
• 8. Stanbury F., P., Whitakar A., and Hall J., S., Principles of fermentation technology, 2nd edition, Aditya books Ltd., New Delhi R17 B. PHARMACY III YEAR