JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

This subject is designed to impart the knowledge on preclinical evaluation of drugs

and recent experimental techniques in the drug discovery and development. The subject content helps the student to understand the maintenance of laboratory animals as per the guidelines.

Course Outcomes

Upon completion of the course the student shall be able to,

• Appraise the regulations and ethical requirement for the usage of experimental animals.
• Describe the various animals and newer screening methods used in the drug discovery
• Understand the Research methodology to be followed Bio-statistical data interpretation of the assays

UNIT - I

Laboratory Animals: Description and applications of different species and strains of animals. Popular transgenic and mutant animals. Techniques for collection of blood and common routes of drug administration in laboratory animals, Techniques of blood collection and euthanasia

UNIT - II

Preclinical screening models: Dose selection, calculation and conversions, preparation of drug solution/suspensions, grouping of animals and importance of sham negative and positive control groups

UNIT - III

Preclinical screening models: for ANS activity, sympathomimetics, sympatholytics, parasympathomimetics, parasympatholytics and skeletal muscle relaxants.

UNIT - IV

Preclinical screening models for diuretics, anticoagulants and anticancer activities

UNIT - V

Research methodology and Bio-statistics, Selection of research topic, review of literature, research hypothesis and study design, Interpretation using Student’t’ test and One-way ANOVA. Graphical representation of data.

Recommended Books (Latest Editions):

• 1. Biological standardization by J.H. Burn D.J. Finney and I.G. Goodwin.
• 2. Screening methods in Pharmacology by Robert Turner. A.
• 3. Methods in Pharmacology by Arnold Schwartz.
• 4. Pharmacological screening methods and Toxicology by A Srinivasa Rao and N. Bhagya Lakshmi
• 5. Fundamentals of experimental Pharmacology by M. N. Ghosh.
• 6. Experimental Pharmacology for undergraduates by M C Prabhakara.
• 7. Drug discovery and Evaluation by Vogel H. G.
• 8. Experimental Pharmacology by R. K. Goyal.
• 9. Preclinical evaluation of new drugs by S.K. Gupta.
• 10. Handbook of Experimental Pharmacology, S K. Kulkarni.
• 11. Practical Pharmacology and Clinical Pharmacy, S K. Kulkarni, 3rd Edition.
• 12. Screening Methods in Pharmacology, Robert A. Turner. R17 B. PHARMACY IV YEAR