JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Overview

Scope: Biochemistry deals with complete understanding of the molecular levels of thechemical process associated with living cells. The scope of the subject is providing biochemical facts and the principles to understand metabolism of nutrient molecules in physiological and pathological conditions. It is also emphasizing on genetic organization of mammalian genome and hetero & autocatalytic functions of DNA.

Course Objectives

Upon completion of course, student shell able to

• Understand the catalytic role of enzymes, importance of enzyme inhibitors in design of new drugs, therapeutic and diagnostic applications of enzymes.
• Understand the metabolism of nutrient molecules in physiological and pathological conditions.
• Understand the genetic organization of mammalian genome and functions of DNA in the synthesis of RNAs and proteins.

UNIT - I

Carbohydrate metabolism Glycolysis – Pathway, energetics and significance Citric acid cycle- Pathway, energetics and significance HMP shunt and its significance; Glucose-6-Phosphate dehydrogenase (G6PD) deficiency Glycogen metabolism Pathways and glycogen storage diseases (GSD) Gluconeogenesis- Pathway and its significance Hormonal regulation of blood glucose level and Diabetes mellitus Biological oxidation Electron transport chain (ETC) and its mechanism. Oxidative phosphorylation & its mechanism and substrate level phosphorylation, Inhibitors ETC and oxidative phosphorylation/Uncouplers

UNIT - II

Lipid metabolism â-Oxidation of saturated fatty acid (Palmitic acid) Formation and utilization of ketone bodies; ketoacidosis De novo synthesis of fatty acids (Palmitic acid) Biological significance of cholesterol and conversion of cholesterol into bile acids, steroid hormone and vitamin D Disorders of lipid metabolism: Hypercholesterolemia, atherosclerosis, fatty liver and obesity.

UNIT - III

Amino acid metabolism General reactions of amino acid metabolism: Transamination, deamination & decarboxylation, urea cycle and its disorders Catabolism of phenylalanine and tyrosine and their metabolic disorders (Phenyketonuria, Albinism, alkeptonuria, tyrosinemia) Synthesis and significance of biological substances; 5-HT, melatonin, dopamine, noradrenaline, adrenaline Catabolism of heme; hyperbilirubinemia and jaundice

UNIT - IV

Nucleic acid metabolism and genetic information transfer Biosynthesis of purine and pyrimidine nucleotides Catabolism of purine nucleotides and Hyperuricemia and Gout disease Organization of mammalian genome Structure of DNA and RNA and their functions DNA replication (semi conservative model) Transcription or RNA synthesis Genetic code, Translation or Protein synthesis and inhibitors

UNIT - V

Enzymes Introduction, properties, nomenclature, and IUB classification of enzymes Enzyme kinetics (Michaelis plot, Line Weaver Burke plot)

Enzyme inhibitors with examples Regulation of enzymes: enzyme induction and repression, allosteric enzymes regulation Therapeutic and diagnostic applications of enzymes and isoenzymes Coenzymes –Structure and biochemical functions

TEXT BOOKS

(Latest Editions)

• 1. Principles of Biochemistry by Lehninger
• 2. Algarsamy V. Pharmaceutical Biochemistry, Pharma Med Press.
• 3. Harper’s Biochemistry by Robert K. Murry, Daryl K. Granner and Victor W. Rodwell.
• 4. Biochemistry by Stryer.
• 5. Biochemistry by D. Satyanarayan and U. Chakrapani
• 6. Textbook of Biochemistry by Rama Rao.
• 7. Textbook of Biochemistry by Deb.
• 8. Outlines of Biochemistry by Conn and Stumpf
• 9. Introduction of Practical Biochemistry by David T. Plummer. (3rd Edition)