JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objective

The subject imparts basic knowledge of animal cell culture. This information will make the student Competent in various cell culture techniques and their applications.

Course Outcome

Upon completion of the course, the student shall be able to:

Explain the various types of cell cultures, their requirements and advantages Appreciate the importance of the bioreactor, cell lines and their applications Explain various culture, preservation and maintenance techniques Explain various IVF techniques, embryo cultures and gene transfer Appreciate the importance of the role embryo culture in and its applications

UNIT - I

Introduction to Animal Biotechnology and its applications: History and scope of animal cell and tissue culture, Advantages and disadvantages of tissue culture, Laboratory facilities for tissue culture. Primary and secondary cell lines cell culture environment, Safety measures laminar hood,

UNIT - II

Basic tissue culture techniques, various types of cultures, Bioreactors, Common cell lines and aseptic methods, Culture media, maintenance and preservation of cell cultures, freezing media, treatment of substrate surfaces.

UNIT - III

Feeder layers on substrate, gas phase for tissue culture, Culture media for cells and tissues, Culture procedures, Disaggregation (enzymatic and mechanical) of tissue and primary culture

UNIT - IV

Cultured cells and evolution of cell lines, Maintenance of culture-cell lines, Tissue culture (slide, flask and test tube cultures), Organ culture, Whole embryo culture, Tissue engineering (artificial skin and artificial cartilage). Cell cultures as a source of valuable products

UNIT - V

In Vitro Fertilization & Transgenic Animals In vitro fertilization (IVF) in humans; embryo transfer (ET) in humans; superovulation, IVF and embryo culture in farm animals (e.g. cow); embryo transfer in cattle, Gene transfer or transfection (using eggs and cultured stem cells); targeted gene transfer; transgenic animals. (mice, sheep, pigs, rabbits, goats, cows, fish).

REFERENCE BOOKS:

• 1. Introduction to Biotechnology, P.K. Gupta, Kalyani Publishers, second edition.
• 2. Introduction to plant Biotechnology, H.S.Chawala, second ed., PHI
• 3. Plant Biotechnology – P. C. Trivedi
• 4. Applied Plant Biotechnology – Ignacimuthu
• 5. Animal Biotechnology – Babinnk and Philips.
• 6. Biotechnology – B. D. Singh.
• 7. Plant Tissue Culture – S.S. Bhojwani, M.K. Razdan.
• 8. Biotechnology Fundamentals and Applications – Purohis S S
• 9. Biotechnology in the Welfare of Mankind – Ali Khan M.Pharm. I Semester (PHARMACOGNOSY)