JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Overview

List of experiments: Methods of extraction: Preparation of extracts of organized crude drugs / Herbs by successive solvent extraction method to record the percentage yield and physical status of the respective extracts and for subjecting them to phytochemical screening. Detection of Phytoconstituents by test tubes and TLC methods, such as

• a. Alkaloids, b. Steroids, Triterpenoids and their glycosides and saponins,
• c. Anthracene glycosides d. Flavanoids and their glycosides
• e. Coumarins f. Tannins
• a. Identification of alkaloids in a mixture by TLC e.g. Atropine, Caffeine, Ergot, Piperine, Quinine, Reserpine, Strychnine and Brucine
• b. Color reactions of different groups of alkaloids. Isolation of the following Phytoconstituents Caffeine from Tea Caffeine from marketed product Strychnine and Brucine from Nux-Vomica by Column chromatography. Piperine from black pepper Citric acid from Lemon Nicotine from Tobacco Pectin from Orange peels Detection, extraction, and estimation of volatile oils by Clevenger’s method (Hydrodistillation method), TLC of volatile oils and their pure constituents. Isolation of starches from potatoes and rice Isolation of Bixin from Bixa orellana Isolation of Lawsone from Henna Preparation of Curcuminoids Identification of bioactive constituents from plant extracts M.Pharm. I Semester (PHARMACOGNOSY)