JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

This subject deals with various hyphenated analytical instrumental techniques

for identification, characterization and quantification of drugs. Instruments dealt are LC-MS, GC-MS, and hyphenated techniques.

Course Outcome

By the completion of topics the students will come out with the thorough

knowledge of various spectral aspects of X-Ray, IR, SEM, ORD etc which help them in further projects works and also industrial opportunities.

UNIT - I

X-Ray diffraction methods: Origin of X-rays, basic aspects of crystals, X-ray crystallography, miller indices, rotating crystal techniques, single crystal diffraction, powder diffraction, structural elucidation and applications.

UNIT - II

• a. Biochromatography: Size exclusion chromatography, ion exchange chromatography, ion pair chromatography, affinity chromatography general principles, stationary phases and mobile phases.
• b. Super critical fluid chromatography: Principles, instrumentation, pharmaceutical applications.

UNIT - III

Capillary Electrophoresis: Overview of CE in pharmaceutical analysis, basic configuration, CE characteristics, principles of CE, methods and modes of CE. General considerations and method development in CE,

UNIT - IV

• a. DSC: Principle, thermal transitions, instrumentation (Heat flux and power- compensation designs), Modulated DSC, Hyper DSC, experimental parameters (sample preparation, experimental conditions, calibration, heating and cooling rates, resolution, Sources of errors) and their influence, advantages and disadvantages, pharmaceutical applications.
• b. DTA: Principle, instrumentation, advantage and disadvantage, pharmaceutical application, derivative differential thermal analysis (DDTA).
• c. TGA: Principle, instrumentation, factors affecting results, advantages and disadvantages, pharmaceutical application.

UNIT - V

Scanning electron microscope (SEM): Principles, Instrumentation and applications. Optical Rotatory Dispersion (ORD), Circular Dichroism, Cotton effect, Octane rule and applications.

REFERENCES:

• 1. Instrumental Methods of Chemical Analysis by B.K Sharma
• 2. Organic spectroscopy by Y.R Sharma
• 3. A Text book of Pharmaceutical Analysis by Kerrenth A. Connors
• 4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
• 5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
• 6. Organic Chemistry by I. L. Finar
• 7. Organic spectroscopy by William Kemp
• 8. Quantitative Analysis of Drugs by D. C. Garrett
• 9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
• 10. Spectrophotometric identification of Organic Compounds by Silverstein
• 11. HPTLC by P.D. Seth M.Pharm I Year II Sem (Pharmaceutical Analysis)