JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

This subject deals with various hyphenated analytical instrumental techniques

for identification, characterization and quantification of drugs. Instruments dealt are LC-MS, GC-MS, and hyphenated techniques.

Course Outcome

By the completion of topics the students will come out with the thorough

knowledge of various electrochemical methods, flourimetry, AAS, RIA, ELISA etc. which help them in further projects works and also industrial opportunities

UNIT - I

Polarography – Principle, Ilkovic equation, construction and working of dropping mercury electrode and rotating platinum electrode, applications. Amperometry - Principles, instrumentation and applications including amperometric titrations.

UNIT - II

• a. Potentiometry – Electrochemical cell, construction and working of reference (Standard hydrogen, silver chloride electrode and calomel electrode) and indicator electrodes (metal electrodes and glass electrode), methods to determine end point of potentiometric titration and applications.
• b. Conductometry– Introduction, Conductivity cell, Conductometric titrations, applications

UNIT - III

Spectroflourimetry: Theory of Fluorescence, Factors affecting fluorescence (Characteristics of drugs that can be analyzed by flourimetry), Quenchers, Instrumentation and Applications of fluorescence spectrophotometer.

UNIT - IV

Flame emission spectroscopy and Atomic absorption spectroscopy: Principle, Instrumentation, Interferences and Applications.

UNIT - V

• a. Radio chemical methods including RIA: Radio Active Isotopes, tagging of compounds, Labeled Reagents, Isotope dilution Analysis, Scintillation counter, RIA.
• b. ELISA: Principle, types and application of ELISA

REFERENCES:

• 1. Instrumental Methods of Chemical Analysis by B.K Sharma
• 2. Organic spectroscopy by Y.R Sharma
• 3. A Text book of Pharmaceutical Analysis by Kerrenth A. Connors
• 4. Vogel’s Text book of Quantitative Chemical Analysis by A.I. Vogel
• 5. Practical Pharmaceutical Chemistry by A.H. Beckett and J.B. Stenlake
• 6. Organic Chemistry by I. L. Finar
• 7. Organic spectroscopy by William Kemp
• 8. Quantitative Analysis of Drugs by D. C. Garrett
• 9. Quantitative Analysis of Drugs in Pharmaceutical Formulations by P. D. Sethi
• 10. Spectrophotometric identification of Organic Compounds by Silverstein
• 11. HPTLC by P.D. Seth M.Pharm I Year II Sem (Pharmaceutical Analysis)