JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objective

This course is designed to impart basic knowledge and skills that are required

animals and their regulatory requirements. The students will know about screening programmes, preclinical and clinical models to perform activities.

Course Outcome

Upon completion of this course it is expected that students shall be able to:

• Understand the care and handling experimental animals
• Understand drug rules and regulations for conducting animal studies
• Know about preclinical & clinical studies of different ANS drugs and their models.

UNIT - I

Care, handling and breeding techniques of laboratory animals. Regulations for laboratory animal care and ethical requirement. Knowledge of the CPCSEA proforma for performing experiments on animals.

UNIT - II

Organization of preclinical screening programme (Blind screening)

UNIT - III

Drug Discovery Process: Principles, techniques and strategies used in drug discovery. High throughput screening, human genomics.

UNIT - IV

Preclinical and clinical models employed in the screening of new drugs belonging to following categories.

• 1. Drugs acting on Autonomic nervous system: Sympathomimetics, Parasympathomimetics, Anticholinesterages, anticholinergics, adrenolytics. Muscle relaxants (peripheral)
• 2. Cardiovascular Pharmacology: Cardiac glycosides, antiarrhythmics, antihypertensives, antiatherosclerotics.
• 3. Screening of free radical scavenging activity
• 4. Immunopharmacology: Specific (Cell and humoral mediated) and non-specific methods.
• 5. Drugs for metabolic disorders: Anti-diabetic agents, Hepatoprotective agents, Anti- hyperlipidemic agents

UNIT - V

Principles of Toxicological evaluations, ED50, LD50 and TD values, acute, sub-acute and chronic toxicity studies.

REFERENCE BOOKS:

• 1. Biological standardization by J. H. Burn D.J. Finney and I.G. Goodwin
• 2. Screening methods in Pharmacology by Robert Turner. A
• 3. Evaluation of drugs activities by Laurence and Bachrach
• 4. Methods in Pharmacology by Arnold Schwartz.
• 5. Fundamentals of experimental Pharmacology by M. N. Ghosh
• 6. Pharmacological experiment on intact preparations by Churchill Livingstone
• 7. Drug discovery and Evaluation by Vogel H.G.
• 8. Experimental Pharmacology by R. K. Goyal.
• 9. Preclinical evaluation of new drugs by S. K. Gupta
• 10. Pharmacological screening methods and Toxicology by A. Srinivasa Rao
• 11. Handbook of Experimental Pharmacology, S K. Kulkarni
• 12. Practical Pharmacology and Clinical Pharmacy, S K. Kulkarni, 3rd Edition.
• 13. David R. Gross. Animal Models in Cardiovascular Research, 2nd Edition, Kluwer Academic Publishers, London, UK.
• 14. Screening Methods in Pharmacology, Robert A. Turner.
• 15. Rodents for Pharmacological Experiments, Dr. Tapan Kumar chatterjee.
• 16. Practical Manual of Experimental and Clinical Pharmacology by Bikash Medhi (Author), Ajay Prakash (Author) M. Pharm I Year I Sem (PHARMACY PRACTICE)