JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objectives

The subject imparts a fundamental knowledge on the structure and functions of

cellular components and help to understand the interaction of these components with drugs. This information will further help the student to apply the knowledge in drug discovery process.

Course Outcomes

Upon completion of the course, the student shall be able to, Explain the receptor

signal transduction processes. Explain the molecular pathways affected by drugs. Appreciate the applicability of molecular pharmacology and biomarkers in drug discovery process. Demonstrate molecular biology techniques as applicable for pharmacology

UNIT - I

Cell biology Structure and functions of cell and its organelles Genome organization. Gene expression and its regulation, importance of siRNA and micro RNA, gene mapping and gene sequencing Cell cycles and its regulation. Cell death– events, regulators, intrinsic and extrinsic pathways of apoptosis. Necrosis and autophagy.

UNIT - II

Cell signaling Intercellular and intracellular signaling pathways. Classification of receptor family and molecular structure ligand gated ion channels; G-protein coupled receptors, tyrosine kinase receptors and nuclear receptors. Secondary messengers: cyclic AMP, cyclic GMP, calcium ion, inositol 1,4,5- trisphosphate, (IP3), NO, and diacylglycerol. Detailed study of following intracellular signaling pathways: cyclic AMP signaling pathway, mitogen-activated protein kinase (MAPK) signaling, Janus kinase (JAK)/signal transducer and activator of transcription (STAT) signaling pathway.

UNIT - III

Principles and applications of genomic and proteomic tools DNA electrophoresis, PCR (reverse transcription and real time), Gene sequencing, micro array technique, SDS page, ELISA and western blotting, Recombinant DNA technology and gene therapy Basic principles of recombinant DNA technology-Restriction enzymes, various types of vectors. Applications of recombinant DNA technology. Gene therapy- Various types of gene transfer techniques, clinical applications and recent advances in gene therapy.

UNIT - IV

Pharmacogenomics Gene mapping and cloning of disease gene. Genetic variation and its role in health/ pharmacology Polymorphisms affecting drug metabolism Genetic variation in drug transporters Genetic variation in G protein coupled receptors Applications of proteomics science: Genomics, proteomics, metabolomics, functionomics, nutrigenomics Immunotherapeutics Types of immunotherapeutics, humanisation antibody therapy, Immunotherapeutics in clinical practice

UNIT - V

Cell culture techniques Basic equipment used in cell culture lab. Cell culture media, various types of cell culture, general procedure for cell cultures; isolation of cells, subculture, cryopreservation, characterization of cells and their application. Principles and applications of cell viability assays, glucose uptake assay, Calcium influx assays Principles and applications of flow cytometry b. Biosimilars

REFERENCE BOOKS:

• 1. The Cell, A Molecular Approach. Geoffrey M Cooper.
• 2. Cellular and Molecular Pharmacology by Amteshwar Singh Jaggi, Pharmamed Press
• 3. Pharmacogenomics: The Search for Individualized Therapies. Edited by J. Licinio and M -L. Wong
• 4. Handbook of Cell Signaling (Second Edition) Edited by Ralph A. et.al
• 5. Molecular Pharmacology: From DNA to Drug Discovery. John Dickenson et.al
• 6. Basic Cell Culture protocols by Cheril D. Helgason and Cindy L. Miller
• 7. Basic Cell Culture (Practical Approach) by J. M. Davis (Editor)
• 8. Animal Cell Culture: A Practical Approach by John R. Masters (Editor)
• 9. Current protocols in molecular biology vol I to VI edited by Frederick M. Ausuvel et la. M.Pharm I Year II Sem (PHARMACY PRACTICE)