JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objective

The topics are designed to help the students to get exposed to various techniques of

plant tissue culture. Use the biotechnological techniques for obtaining and improving the quality of natural products/medicinal plants

Course Outcome

Students will gain the knowledge about various strategies of plant tissue culture and

students will gain knowledge about various secondary metabolites produced by plant tissue culture.

UNIT - I

Introduction to Plant biotechnology: Historical perspectives, Laboratory Organization, Maintenance of asepsis in tissue culture, Nutritional requirements, Media preparation, Explant preparation, Establishment of Aseptic cultures- Initiation and maintenance of callus and suspension culture. Biotechnological applications of Plant Tissue culture in pharmacy and allied fields.

UNIT - II

Different tissue culture techniques: Types and techniques of plant tissue culture, growth parameters, Organogenesis and embryogenesis, Protoplast isolation and culture.

UNIT - III

Micro propagation of medicinal and aromatic plants, Immobilization techniques of plant cell & Secondary Metabolite Production. Precursors and elicitors on production of secondary metabolites, Cryopreservation of germ plasm, synthetic seed.

UNIT - IV

Biotransformation and Trangenesis: Biotransformation of Plant Cell Culture and its importance in secondary metabolite production. Bioreactors for pilot and large scale cultures of plant cells. Hairy root cultures and their applications.

UNIT - V

Secondary metabolism in tissue cultures with emphasis on production of medicinal agents- Production of Secondary metabolites from callus culture and suspension culture with emphasis on production of biomedicinals like- Ajmalicine, Shikonin, Carotenoids and Rosemarinic acid.

TEXT BOOKS:

• 1. Medicinal plant biotechnology by Ciddi Veeresham
• 2. Pharmaceuticals biotechnology by S.P. Vyas & V.K. Dixit
• 3. Pharmacognosy and Pharmacobiotechnology by Ashutoshkar
• 4. Introduction to plant tissue culture by M. K. Razadam
• 5. Plant tissue culture by Street
• 6. Pharmacognosy and Phytochemistry, A Comprehensive Approach 2nd Edition by S.L. Doore, S.S Khadabadi and B.A. Baviskar

REFERENCE BOOKS:

• 1. Plant Tissue Culture by Bhojwani
• 2. Molecular Biology and Biotechnology by J.M. Walker and E.D. Gingo
• 3. Advanced methods in Plant breeding and Biotechnology by David R Mirray
• 4. Biotechnology by Purohit and Mathur, Agro-Bio, 3rd revised edition.
• 5. Biotechnological applications to tissue culture by Shargool, Peter D, Shargoal, CKC Press.
• 6. Pharmacognosy by Varo E. Tyler, Lynn R. Brady and James E. Robberrt, That Tjen, NG M.Pharm I Year II Sem (PHARMACOGNOSY)