JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Overview

List of experiments:

• 1. Methods of extraction: Preparation of extracts of organized crude drugs / Herbs by successive solvent extraction method to record the percentage yield and physical status of the respective extracts and for subjecting them to phytochemical screening.
• 2. Detection of Phytoconstituents by test tubes and TLC methods, such as
• a. Alkaloids, b. Steroids, Triterpenoids and their glycosides and saponins,
• c. Anthracene glycosides d. Flavanoids and their glycosides
• e. Coumarins f. Tannins
• 3. a. Identification of alkaloids in a mixture by TLC e.g. Atropine, Caffeine, Ergot, Piperine, Quinine, Reserpine, Strychnine and Brucine
• b. Color reactions of different groups of alkaloids.
• 4. Isolation of the following Phytoconstituents
• a. Caffeine from Tea
• b. Caffeine from marketed product
• c. Strychnine and Brucine from Nux-Vomica by Column chromatography.
• d. Piperine from black pepper
• e. Citric acid from Lemon
• f. Nicotine from Tobacco
• g. Pectin from Orange peels
• 5. Detection, extraction, and estimation of volatile oils by Clevenger’s method (Hydrodistillation method), TLC of volatile oils and their pure constituents.
• 6. Isolation of starches from potatoes and rice
• 7. Isolation of Bixin from Bixa orellana
• 8. Isolation of Lawsone from Henna
• 9. Preparation of Curcuminoids
• 10. Identification of bioactive constituents from plant extracts M.Pharm I Year I Sem (PHARMACOGNOSY)