JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

Course Objective

To impart knowledge on application of biotechnological concepts to minimize and

mitigate environmental pollution.

Course Outcome:

• An outlook on biotechnological concepts and biodegradation.
• Knowledge on the usage of biotechnology like enzyme/cell/DNA technology etc. for mitigation of environmental pollution.

UNIT - I

Environmental Biotechnology – Principles and concepts –usefulness to mankind. Degradation of high concentrated toxic pollutants – halogenated non halogenated, petroleum hydrocarbons, metals- Mechanisms of detoxification – oxidation – dehalogenation- biotransformation of metals – biodegradation of solid wastes.

UNIT - II

Biotechnological remedies for environmental pollution- decontamination of groundwater- bioremediation – Production of proteins- biofertilizers – Physical, chemical and microbiological factors of composting – health risk- pathogens- odor management.

UNIT - III

Mircobial cell/enzyme technology – adapted microorganisms – biological removal of nutrients – algal biotechnology – extra cellular polymers – Biogas technology.

UNIT - IV

Concept of rDNA technology – expression vectors – cloning of DNA mutation – construction of microbial strains – radioactive probes – protoplast fusion technology applications.

UNIT - V

Environmental effects and ethics of microbial technology – genetically engineered organisms – Microbial containment – Risk assessment.

TEXT BOOKS:

• 1. Chaudhury, G.R. Biological degradation and bioremediation of toxic chemicals, Dioscorides Press, Oregon, 1994.
• 2. Martin. A.M. Biological degradation of wastes, Elsevier Applied Science, London, 1991.
• 3. Blaine Metting .F (Jr.,) Soil Microbiology Ecology, Marcel Dekker Inc., 1993. M. Tech – I Year – II Sem. (Envir. Engg.)