JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD

MICROBIOLOGY LAB

M.Tech I Year I Semester (R22)

List of Experiments:

  • 1. Lab safety method and Regulations.
  • 2. Principles and methods of sterilization.
  • 3. Preparation of media: Nutrient broth, Nutrient agar, slants, soft agar
  • 4. Pure culture technique – Pour plate, Spread plate & Streak plate.
  • 5. Measurement of microbes – Micrometry.
  • 6. Motility determination – Hanging drop method.
  • 7. Enumeration of bacterial / yeast cells – Viable count, Total count.
  • 8. Enumeration of bacteria / fungi from environmental samples – Direct and Indirect methods (Haemocytometer & Total viable counts).
  • 9. Staining methods: Simple, Negative, Acid fast, Gram staining, Spore, Flagella, Capsule and Metachromatic granular staining. Staining of fungus – Lacto phenol cotton blue staining.
  • 10. Measurement of growth – Direct count, Viable count – Growth curve, Determination of growth rate and generation time.

REFERENCES:

  • 1. Cappuccino J.G, N.Sherman, (2002), Microbiology: A Laboratory Manual, Addison– Wesley.
  • 2. Atlas R. M, A. E. Brown and L. C. Parks, (1995), Laboratory Manual of Experimental Microbiology, Mosby, St. Louis.
  • 3. Kannan K., (2002), Laboratory Manual in General Microbiology, Panima Publishers. M. Tech – I Year – I Sem. (Env. Engg.)

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