JNTU
JNTU B. Pharmacy III PHARMACEUTICAL BIOTECHNOLOGY Previous Question Paper (July - 2023 - R17)
Note: i) Question paper consists of Part A, Part B. ii) Part A is compulsory, which carries 25 marks. In Part A, answer all questions. iii) In Part B, Answer any one question from each unit. Each question carries 10 marks and may have a, b as sub questions.
Part A
List the methods of enzyme immobilization.
Answer
The main methods of enzyme immobilization are: 1) Adsorption, 2) Covalent bonding, 3) Entrapment (matrix or membrane), and 4) Cross-linking.
Briefly highlight the basic principles of genetic engineering.
Answer
Genetic engineering is based on the isolation of a specific gene (gene of interest), its insertion into a suitable vector to form recombinant DNA, and the introduction of this construct into a host cell for cloning and expression of the desired protein.
Write the functions of DNA ligase.
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What are restriction endonucleases? Mention the different types.
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What is Hybridoma technology?
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Define hypersensitivity. Enumerate different types.
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Define microbial biotransformation. Give examples.
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Enumerate the steps involved in gene transfer and their significance.
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List the microorganisms used in the production of vitamin B12.
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Write a note on various controls in large scale fermenters.
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Part B
Explain working and applications of biosensors in Pharmaceutical Industries.
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Outline the production of Amylase.
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Outline the production of Peroxidase.
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Explain the role of biotechnology in pharmaceuticals and enlist the applications of enzyme immobilization.
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Explain the production of Interferons with a neat labelled diagram.
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Describe the application of PCR in r-DNA technology.
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Describe the application of plasmids and shuttle vectors in r-DNA technology.
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Explain in brief the technique of preparation of r-DNA.
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Describe the general method of production of bacterial toxoids.
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Discuss the structure and function of MHC.
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Discuss the normal serum level, half-life and application of the five different types of Immunoglobulins.
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Describe the general method of production of immune sera.
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What are the distinguishing characteristics of each of the three type of gene transfer in Bacteria.
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Explain the procedure of involved in direct ELISA technique with diagram.
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Explain the procedure involved in Western blotting technique with diagram.
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Discuss the genetic organization of Eukaryotes and Prokaryotes.
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Explain the major differences among various bioreactor types?
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Discuss briefly the largescale production of Griseofulvin.
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Explain the steps and unit operations involved in downstream processing.
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Discuss the largescale production of Glutamic acid.
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