Jawaharlal Nehru Technological University Hyderabad
Jawaharlal Nehru Technological University Hyderabad B.Pharmacy II PHARMACEUTICAL MICROBIOLOGY Previous Question Paper (March - 2024 - R17)
Note: i) Question paper consists of Part A, Part B. ii) Part A is compulsory, which carries 25 marks. In Part A, answer all questions. iii) In Part B, Answer any one question from each unit. Each question carries 10 marks and may have a, b as sub questions.
PART - A
Explain the following:
Structure of Bacteria.
Answer
A bacterial cell consists of an outer cell envelope (capsule, cell wall, and plasma membrane), cytoplasm containing ribosomes, a nucleoid (circular DNA), and appendages such as flagella, pili, or fimbriae.
Growth curve
Answer
The bacterial growth curve represents the population growth of bacteria in a closed system over time, consisting of four distinct phases: the lag phase (adaptation), log/exponential phase (rapid division), stationary phase (growth equals death), and decline/death phase (nutrient depletion).
Sterilization
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Staining techniques
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Antiseptics
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Ophthalmic Sterility products
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Laminar air flow
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Different sources of Contamination
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Antimicrobial Agents.
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Primary Culture
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PART - B
Explain the differences between prokaryotes and Eukaryotes.
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Describe the structure, principle and functions of Electron Microscopy.
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Write about the nutritional requirements and raw materials used for Culture Media Preparation.
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Explain about the quantitative measurements of Bacterial growth (Total and Viable count).
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Explain about the principle, applications of physical, Chemical and mechanical methods of sterilization
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Write about the identification of bacteria by using staining techniques.
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Explain about the principle, procedure, merits and demerits of Sterilization
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Write about the Sterility Indicators.
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Explain about the classification, reproduction and cultivation of Fungi.
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Describe the classification and mode of action of disinfectants.
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Explain about the evaluation of bactericidal and bacteriostatic actions of sterility Testing products.
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Write about the factors influencing disinfection, antiseptics and their Evaluation.
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Explain about the design of aseptic area and add a note on Laminar flow equipments
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Write about the classification of clean area and add a note on methods of preservation.
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Explain about the assessment of a new antibiotic and testing of antimicrobial activity of new substances.
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Write about the methods for Standardization of Antibiotics, Vitamins and Amino acids.
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What are the different types of spoilages and add a note on Sources and types of Microbial contaminants.
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Define cell culture and add a note on Growth of animal cells in culture.
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Write about the evaluation of microbial stability of formulations.
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Explain about the general procedure for cell culture and add a note on established and transformed cell cultures.
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